Relationship between the injury time and DNA fragmentation and its quantity in rat brain / 法医学杂志

ABSTRACT

OBJECTIVE@#To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.@*METHODS@#The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.@*RESULTS@#With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.@*CONCLUSION@#TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.