The degradation of housekeeping mRNA in dead rats by real-time RT-PCR / 法医学杂志
Journal of Forensic Medicine
; (6): 33-36, 2009.
Article
ي Zh
| WPRIM
| ID: wpr-983438
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE@#To explore the feasibility of real-time RT-PCR of housekeeping mRNA degradation in dead rats in order to seek new suitable techniques for post-mortem interval (PMI).@*METHODS@#The levels of GAPDH mRNA and beta-actin mRNA in the brain and spleen of the rats were measured at different times after death by the SYBR Green I real-time RT-PCR. The relative mRNA level was indicated with the Ct value, then the relationship between the Ct value and PMI were analyzed and the corresponding regression equation was obtained.@*RESULTS@#The Ct values of GAPDH mRNA and beta-actin mRNA by SYBR Green I real time RT-PCR correlated well with the PMI.@*CONCLUSION@#The SYBR Green I real-time RT-PCR is a reliable technique for studying mRNA degradation. Adoption of the housekeeping genes eliminates systematical errors of other genes which have individual difference. As an objective and dynamic indication, Ct value has a good correlation with PMI, and could be applied for estimating PMI, especially in late period.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Postmortem Changes
/
Time Factors
/
RNA, Messenger
/
Actins
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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RNA Stability
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Glyceraldehyde-3-Phosphate Dehydrogenases
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Forensic Medicine
السنة:
2009
نوع:
Article