Identification of Vaginal Fluid Using Microbial Signatures / 法医学杂志
Journal of Forensic Medicine
; (6): 254-256, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-984841
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVES@#To investigate the specific microbial signatures in vaginal fluid.@*METHODS@#Vaginal fluid (16 samples), saliva (16 samples), feces (16 samples), semen (8 samples), peripheral blood (8 samples), urine (5 samples), and nasal secretion (4 samples) were collected respectively. The 16S rRNA genes of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were amplified. PCR production was detected via a 3130xl Genetic Analyzer.@*RESULTS@#The detected number of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. Lactobacillus crispatus and Lactobacillus jensenii existed specifically in vaginal fluid.@*CONCLUSIONS@#There is a potential application value to detect Lactobacillus crispatus and Lactobacillus jensenii for the identification of vaginal fluid.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Saliva
/
Semen
/
Vagina
/
Blood
/
Body Fluids
/
RNA, Ribosomal, 16S
/
Polymerase Chain Reaction
/
Actinobacteria
/
Feces
/
Genes, Bacterial
المحددات:
Female
/
Humans
اللغة:
Zh
مجلة:
Journal of Forensic Medicine
السنة:
2016
نوع:
Article