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Comparison of apical periodontitis repair in endodontic treatment with calcium hydroxide-dressing and aPDT
Silva, Lea Assed Bezerra da; Lopes, Zobélia Maria de Souza; Sá, Rafaela Cardoso de; NOVAES JÚNIOR, Arthur Belém; Romualdo, Priscilla Coutinho; Lucisano, Marília Pacífico; Nelson-Filho, Paulo; Silva, Raquel Assed Bezerra da.
  • Silva, Lea Assed Bezerra da; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Lopes, Zobélia Maria de Souza; Faculdade do Amazonas. Amazonian Institute of Higher Education. Manaus. BR
  • Sá, Rafaela Cardoso de; Faculdade do Amazonas. Amazonian Institute of Higher Education. Manaus. BR
  • NOVAES JÚNIOR, Arthur Belém; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Romualdo, Priscilla Coutinho; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Lucisano, Marília Pacífico; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Nelson-Filho, Paulo; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
  • Silva, Raquel Assed Bezerra da; Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry. Ribeirão Preto. BR
Braz. oral res. (Online) ; 33: e092, 2019. tab, graf
Article in English | LILACS | ID: biblio-1039296
ABSTRACT
Abstract This study evaluated the effect of antimicrobial photodynamic therapy (aPDT) on the endodontic treatment of apical periodontitis (AP). AP was induced in 48 premolars of 6 dogs. After biomechanical preparation, the teeth were divided into 4 groups Calcium-Hydroxide (CH)/120d and CH/180d root canals filled with CH-based dressing for 15 days before obturation; aPDT/120d and aPDT/180d conditioning with phenothiazine photosensitizer (10 mg/mL) for 1 minute and irradiation with diode laser in the same session as obturation. Root filling was performed with AH Plus sealer. After the experimental periods, animals were euthanized and teeth were submitted for histology. HE staining was performed for descriptive analysis of the periapical region, measurement of apical periodontitis and for inflammatory cells, and blood vessels count. Immunohistochemistry was performed for osteopontin (OPN) and alkaline phosphatase (ALP). Data were analyzed statistically by two-way ANOVA and chi-square test (α = 5%). Teeth in Group CH/120d presented only a slightly enlarged periodontal ligament (PL) with advanced repair. Group aPDT/120d presented the PL moderately enlarged, with moderate inflammatory infiltrate and few collagen fibers. The same pattern was observed at 180 days. AP lesions in CH-treated groups were smaller than those in aPDT-treated groups (p < 0.001) with more blood vessels (p < 0.0001), regardless of the evaluation period, without significant differences in the number of inflammatory cells (p > 0.05). CH-treated groups showed significantly more intense immunostaining for ALP and OPN (p < 0.001) in both periods. Although aPDT stimulated angiogenesis and expression of bone formation markers, the two-session endodontic treatment with CH-based dressing promoted better apical periodontitis repair.
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Full text: Available Index: LILACS (Americas) Main subject: Periapical Periodontitis / Photochemotherapy / Root Canal Therapy / Bone Cements / Calcium Hydroxide Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade do Amazonas/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Periapical Periodontitis / Photochemotherapy / Root Canal Therapy / Bone Cements / Calcium Hydroxide Limits: Animals Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade do Amazonas/BR / Universidade de São Paulo/BR