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Biological roles of filamin a in prostate cancer cells
Li, Xue-Chao; Huang, Chuan-Xi; Wu, Shi-Kui; Yu, Lan; Zhou, Guang-Jian; Chen, Li-Jun.
  • Li, Xue-Chao; Chinese PLA General Hospital. the Fifth Medical Center. Department of Urology. Beijing. CN
  • Huang, Chuan-Xi; Hebei University. College of Life Science. Hebei. CN
  • Wu, Shi-Kui; Chinese PLA General Hospital. the Fifth Medical Center. Department of Urology. Beijing. CN
  • Yu, Lan; Beijing Institute of Biotechnology. Laboratory of Medical Molecular Biology. Beijing. CN
  • Zhou, Guang-Jian; Beijing Institute of Biotechnology. Laboratory of Medical Molecular Biology. Beijing. CN
  • Chen, Li-Jun; Chinese PLA General Hospital. the Fifth Medical Center. Department of Urology. Beijing. CN
Int. braz. j. urol ; 45(5): 916-924, Sept.-Dec. 2019. graf
Article in English | LILACS | ID: biblio-1040072
ABSTRACT
ABSTRACT Objective This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa. Materials and Methods A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the suppression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test. Results A cell line model with a low expression of filamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P <0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P <0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P <0.05). Conclusion The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene.
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Full text: Available Index: LILACS (Americas) Main subject: Prostatic Neoplasms / Filamins Type of study: Prognostic study Limits: Humans / Male Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Beijing Institute of Biotechnology/CN / Chinese PLA General Hospital/CN / Hebei University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Prostatic Neoplasms / Filamins Type of study: Prognostic study Limits: Humans / Male Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Beijing Institute of Biotechnology/CN / Chinese PLA General Hospital/CN / Hebei University/CN