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IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Gusmão, Michélia Antônia do Nascimento; Emídio, Nayara Braga; Marconato, Danielle Gomes; Farani, Priscila Silva Grijó; Maniezzi, Luiz Felipe; Coelho, Paulo Marcos Zech; Oliveira, Rodrigo Correa; Vasconcelos, Eveline Gomes; Faria-Pinto, Priscila de.
  • Gusmão, Michélia Antônia do Nascimento; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Emídio, Nayara Braga; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Marconato, Danielle Gomes; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Farani, Priscila Silva Grijó; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Maniezzi, Luiz Felipe; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Coelho, Paulo Marcos Zech; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte. BR
  • Oliveira, Rodrigo Correa; Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte. BR
  • Vasconcelos, Eveline Gomes; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
  • Faria-Pinto, Priscila de; Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora. BR
Rev. Soc. Bras. Med. Trop ; 52: e20180139, 2019. graf
Article in English | LILACS | ID: biblio-1041506
ABSTRACT
Abstract

INTRODUCTION:

High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.

METHODS:

Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.

RESULTS:

Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.

CONCLUSIONS:

Potato apyrase and SmATPDases have IgE-binding epitopes.
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Full text: Available Index: LILACS (Americas) Main subject: Apyrase / Schistosoma mansoni / Schistosomiasis mansoni / Solanum tuberosum / Immunoglobulin E / Antibodies, Helminth / Epitopes Limits: Animals Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR / Universidade Federal de Juiz de Fora/BR

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Full text: Available Index: LILACS (Americas) Main subject: Apyrase / Schistosoma mansoni / Schistosomiasis mansoni / Solanum tuberosum / Immunoglobulin E / Antibodies, Helminth / Epitopes Limits: Animals Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz/BR / Universidade Federal de Juiz de Fora/BR