Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
Rev. bras. entomol
;
62(1): 19-22, Jan.-Mar. 2018. tab, graf
Article
in English
| LILACS
| ID: biblio-1045487
ABSTRACT
ABSTRACT Bombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an effective way to improve resistance to BmNPV and reduce economic losses. A precise resistance-detection method will help to accelerate the breeding process. For this purpose, here we described the individual inoculation method (IIM). Details of the IIM include pathogen BmNPV preparation, mulberry leaf size, pathogen volume, rearing conditions, course of infection, and breeding conditions. Finally, a resistance comparison experiment was performed using the IIM and the traditional group inoculation method (GIM). The incidence of BmNPV infection and the within-group variance results showed that the IIM was more precise and reliable than the GIM.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Rev. bras. entomol
Journal subject:
Biology
Year:
2018
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Sericulture & Agri-food Research Institute/CN
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