Dexmedetomidine attenuates P2X4 and NLRP3 expression in the spine of rats with diabetic neuropathic pain
Acta cir. bras
;
34(11): e201901105, Nov. 2019. graf
Article
in English
| LILACS
| ID: biblio-1054679
ABSTRACT
Abstract Purpose:
To evaluate the effects of Dexmedetomidine (Dex) on spinal pathology and inflammatory factor in a rat model of Diabetic neuropathic pain (DNP).Methods:
The rats were divided into 3 groups (eight in each group) normal group (N group), diabetic neuropathic pain model group (DNP group), and DNP model with dexmedetomidine (Dex group). The rat model of diabetes was established with intraperitoneal streptozotocin (STZ) injections. Nerve cell ultrastructure was evaluated with transmission electron microscopy (TEM). The mechanical withdrawal threshold (MWT) and motor nerve conduction velocity (MNCV) tests documented that DNP rat model was characterized by a decreased pain threshold and nerve conduction velocity.Results:
Dex restored the phenotype of neurocytes, reduced the extent of demyelination and improved MWT and MNCV of DNP-treated rats (P=0.01, P=0.038, respectively). The expression of three pain-and inflammation-associated factors (P2X4, NLRP3, and IL-IP) was significantly upregulated at the protein level in DNP rats, and this change was reversed by Dex administration (P=0.0022, P=0.0092, P=0.0028, respectively).Conclusion:
The P2X4/NLRP3 signaling pathway is implicated in the development and presence of DNP in vivo, and Dex protects from this disorder.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Spine
/
Dexmedetomidine
/
Diabetic Neuropathies
/
Receptors, Purinergic P2X4
/
Adrenergic alpha-2 Receptor Agonists
/
NLR Family, Pyrin Domain-Containing 3 Protein
Type of study:
Evaluation studies
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
Renmin Hospital of Wuhan University/CN
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