Your browser doesn't support javascript.
loading
Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model / Terapia de magneto-hipertermia no modelo de tumor de glioblastoma on-a-Chip
Mamani, Javier Bustamante; Marinho, Bruna Souto; Rego, Gabriel Nery de Albuquerque; Hospital das ClínicasNucci, Mariana Penteado; Alvieri, Fernando; Santos, Ricardo Silva dos; Ferreira, João Victor Matias; Oliveira, Fernando Anselmo de; Gamarra, Lionel Fernel.
  • Mamani, Javier Bustamante; Hospital Israelita Albert Einstein. São Paulo. BR
  • Marinho, Bruna Souto; Hospital Israelita Albert Einstein. São Paulo. BR
  • Rego, Gabriel Nery de Albuquerque; Hospital Israelita Albert Einstein. São Paulo. BR
  • Hospital das ClínicasNucci, Mariana Penteado; Universidade de São Paulo. Faculdade de Medicina. Hospital das ClínicasNucci, Mariana Penteado. São Paulo. BR
  • Alvieri, Fernando; Hospital Israelita Albert Einstein. São Paulo. BR
  • Santos, Ricardo Silva dos; Hospital Israelita Albert Einstein. São Paulo. BR
  • Ferreira, João Victor Matias; Hospital Israelita Albert Einstein. São Paulo. BR
  • Oliveira, Fernando Anselmo de; Hospital Israelita Albert Einstein. São Paulo. BR
  • Gamarra, Lionel Fernel; Hospital Israelita Albert Einstein. São Paulo. BR
Einstein (Säo Paulo) ; 18: eAO4954, 2020. graf
Article in English | LILACS | ID: biblio-1056032
ABSTRACT
ABSTRACT

Objective:

To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device.

Methods:

The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20μL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes.

Results:

Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively.

Conclusion:

The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-a-chip model was effective to produce the total cell lise after 30 minutes of therapy.
RESUMO
RESUMO

Objetivo:

Avaliar a terapia de magneto-hipertermia em modelo de tumor de glioblastoma on-a-Chip.

Métodos:

As nanopartículas magnéticas recobertas com aminosilana foram utilizadas para a terapia da magneto-hipertermia, sendo avaliada a taxa de absorção específica das nanopartículas magnéticas em 300 Gauss e 305kHz. Uma pré-cultura de células C6 foi realizada e, seguidamente, foi feito o cultivo das células 3D no chip. O processo de magneto-hipertermia no chip foi realizado após administração de 20μL de nanopartículas magnéticas (10mgFe/mL), utilizando os parâmetros que geraram o valor da taxa de absorção específica. A eficácia da terapia de magneto-hipertermia foi avaliada pela viabilidade celular por meio dos corantes fluorescentes acetoximetiléster de calceína (492/513nm), para células vivas, e etídio homodímero-1 (526/619nm), para células mortas.

Resultados:

As nanopartículas magnéticas, quando submetidas ao campo magnético alternado (300 Gauss e 305kHz), produziram um valor médio da taxa de absorção específica de 115,4±6,0W/g. A cultura 3D das células C6 avaliada por imagem de microscopia de campo claro mostrou a proliferação e a morfologia das células antes da aplicação da terapia de magneto-hipertermia. As imagens de fluorescência mostraram diminuição da viabilidade das células cultivadas no organ-on-a-Chip em 20% e 100% após 10 e 30 minutos, respectivamente, da aplicação da terapia de magneto-hipertermia.

Conclusão:

O processo terapêutico da magneto-hipertermia no modelo de tumor glioblastoma on-a-chip foi eficaz para produzir lise total das células após 30 minutos de terapia.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Glioblastoma / Cell Culture Techniques / Lab-On-A-Chip Devices / Magnetite Nanoparticles / Hyperthermia, Induced Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Einstein (Säo Paulo) Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Israelita Albert Einstein/BR / Universidade de São Paulo/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Glioblastoma / Cell Culture Techniques / Lab-On-A-Chip Devices / Magnetite Nanoparticles / Hyperthermia, Induced Type of study: Evaluation studies / Prognostic study Limits: Animals Language: English Journal: Einstein (Säo Paulo) Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Israelita Albert Einstein/BR / Universidade de São Paulo/BR