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Vitamin C administration attenuated artemether induced hepatic injury in rats / La aAdministración de vitamina C atenúa la lesión hepática inducida por artemeter en ratas
Eid, Refaat A; Zaki, Mohamed Samir Ahmed; Alghamdi, Mansour A; Sideeg, Abulqasim Mohammed; Ali, Kamal, Z. M; Andarawi, Mohamed; Haidara, Mohamed A.
  • Eid, Refaat A; King Khalid University. College of Medicine. Pathology department. SA
  • Zaki, Mohamed Samir Ahmed; King Khalid University. College of Medicine. Anatomy department. SA
  • Alghamdi, Mansour A; King Khalid University. College of Medicine. Anatomy department. SA
  • Sideeg, Abulqasim Mohammed; King Khalid University. College of Medicine. Anatomy department. SA
  • Ali, Kamal, Z. M; King Khalid University. College of Medicine. Anatomy department. SA
  • Andarawi, Mohamed; King Khalid University. College of Medicine. Pathology department. SA
  • Haidara, Mohamed A; Cairo University. Kasr al-Aini Faculty of Medicine. Physiology department. Cairo. EG
Int. j. morphol ; 38(1): 48-55, Feb. 2020. graf
Article in English | LILACS | ID: biblio-1056396
ABSTRACT
This research was designed to investigate the potential protective effect of vitamin C supplementation against hepatocyte ultrastructural alterations induced by artemether (antimalarial drug) administration. Twenty-four adult male albino rats were used in this study and were divided into four groups (n=6). Group I served as a control and rats in group II administrated artemether (4 mg/kg B.W) orally for three consecutive days. Group III administered artemether plus a low dose of vitamin C (2.86 mg/kg/l water) while group IV received artemether plusa high dose of vitamin C (8.56 mg/kg). At the end of the experimental period (14 days), the harvested liver tissues were examined by transmission electron microscopy (TEM), and blood samples were assayed for biomarkers of liver injury and oxidative stress. Artemether significantly (p<0.05) augmented biomarkers of liver injury such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and oxidative stress such as superoxide dismutase (SOD), Glutathione Peroxidase (GPX), and caused degeneration and damage of the rough endoplasmic reticulum and disrupted mitochondria. The blood sinusoids were also damaged with distortion of their canaliculi. Administration of vitamin C showed improvement of liver biomarkers, and liver parenchyma, especially in a high dose of vitamin C.We concludes that vitamin C is a partial protective agent against artemether-induced liver injury.
RESUMEN
Esta investigación fue diseñada para investigar el posible efecto protector de la vitamina C contra las alteraciones ultraestructurales de los hepatocitos, inducidas por la administración de arteméter (medicamento antipalúdico). En el estudio se utilizaron 24 ratas albinas macho adultas y se dividieron en cuatro grupos (n = 6). El grupo I fue designado como control y las ratas en el grupo II se adminstró Arteméter (4 mg / kg de peso corporal) por vía oral durante tres días consecutivos. En el grupo III se administró arteméter, además de una dosis baja de vitamina C (2,86 mg / kg / l de agua) mientras que el grupo IV recibió arteméter más una dosis alta de vitamina C (8,56 mg / kg). Al final del período experimental (14 días), los tejidos hepáticos recolectados se examinaron por microscopía electrónica de transmisión (MET), y las muestras de sangre se analizaron en busca de biomarcadores de daño hepático y estrés oxidativo. El arteméter aumentó significativamente (p <0,05) los biomarcadores de daño hepático como alanina aminotransferasa (ALT), aspartato aminotransferasa (AST) y estrés oxidativo como superóxido dismutasa (SOD), glutatión peroxidasa (GPX) y causó degeneración y daño de la retículo endoplásmico rugoso y mitocondrias alteradas. Los sinusoides sanguíneos también fueron dañados con la distorsión de sus canalículos. La administración de vitamina C mostró una mejoría de los biomarcadores hepáticos y el parénquima hepático, especialmente en una dosis alta de vitamina C. Concluimos que la vitamina C es un agente protector parcial contra la lesión hepática inducida por arteméter.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Ascorbic Acid / Chemical and Drug Induced Liver Injury, Chronic / Artemether Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA

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Full text: Available Index: LILACS (Americas) Main subject: Ascorbic Acid / Chemical and Drug Induced Liver Injury, Chronic / Artemether Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2020 Type: Article Affiliation country: Egypt / Saudi Arabia Institution/Affiliation country: Cairo University/EG / King Khalid University/SA