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Effects of Rab7 gene up-regulation on renal fibrosis induced by unilateral ureteral obstruction
Xu, Qing; Liu, Lei; Department of Pathology and PathophysiologyYang, Yiqiong; Department of Pathology and PathophysiologyWang, Zhi; Department of Pathology and PathophysiologyCai, Yingying; Hong, Tingting; Department of Pathology and PathophysiologyChen, Pingsheng.
  • Xu, Qing; Affiliated Hospital of Jiangnan University. Department of Pathology. Wuxi. CN
  • Liu, Lei; Zhongshan Hospital, Fudan University. Department of Pathology. Shanghai. CN
  • Department of Pathology and PathophysiologyYang, Yiqiong; Southeast University. School of Medicine. Department of Pathology and PathophysiologyYang, Yiqiong. Nanjing. CN
  • Department of Pathology and PathophysiologyWang, Zhi; Southeast University. School of Medicine. Department of Pathology and PathophysiologyWang, Zhi. Nanjing. CN
  • Department of Pathology and PathophysiologyCai, Yingying; Southeast University. School of Medicine. Department of Pathology and PathophysiologyCai, Yingying. Nanjing. CN
  • Hong, Tingting; Affiliated Hospital of Jiangnan University. Department of Oncology. Wuxi. CN
  • Department of Pathology and PathophysiologyChen, Pingsheng; Southeast University. School of Medicine. Department of Pathology and PathophysiologyChen, Pingsheng. Nanjing. CN
Braz. j. med. biol. res ; 53(4): e9220, 2020. graf
Article in English | LILACS | ID: biblio-1089355
ABSTRACT
Rab7, an important member of the Rab family, is closely related to autophagy, endocytosis, apoptosis, and tumor suppression but few studies have described its association with renal fibrosis. In the early stage, our group studied the effects of Rab7 on production and degradation of extracellular matrix in hypoxic renal tubular epithelial cells. Because cell culture in vitro is different from the environment in vivo, it is urgent to understand the effects in vivo. In our current study, we established a renal fibrosis model in Rab7-knock-in mice (prepared by CRISPR/Cas9 technology) and wild type (WT) C57BL/6 mice using unilateral ureteral obstruction (UUO). Seven and 14 days after UUO, the expression of the Rab7 protein in WT mice, as well as the autophagic activity, renal function, and the degree of renal fibrosis in WT and Rab7-knock-in mice were examined by blood biochemical assay, hematoxylin-eosin and Masson staining, immunohistochemistry, and western blotting. We found that the Rab7 expression in WT mice increased over time. Furthermore, the autophagic activity constantly increased in both groups, although it was higher in the Rab7-knock-in mice than in the WT mice at the same time point. Seven days after UUO, the degree of renal fibrosis was milder in the Rab7-knock-in mice than in the WT mice, but it became more severe 14 days after surgery. Similar results were found for renal function. Therefore, Rab7 suppressed renal fibrosis in mice initially, but eventually it aggravated fibrosis with the activation of autophagy.
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Full text: Available Index: LILACS (Americas) Main subject: Autophagy / Ureteral Obstruction / Rab GTP-Binding Proteins / Kidney / Kidney Diseases Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Hospital of Jiangnan University/CN / Southeast University/CN / Zhongshan Hospital, Fudan University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Autophagy / Ureteral Obstruction / Rab GTP-Binding Proteins / Kidney / Kidney Diseases Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Hospital of Jiangnan University/CN / Southeast University/CN / Zhongshan Hospital, Fudan University/CN