Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection
Rev. Soc. Bras. Med. Trop
;
53: e20190181, 2020. graf
Article
in English
| LILACS
| ID: biblio-1092205
ABSTRACT
Abstract INTRODUCTION:
Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of the host and can reactivate after the primary infection, leading to lytic replication in immunosuppressed patients.METHODS:
This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) to identify HHV-7 serum antibodies and compare its performance with that of an indirect immunofluorescence assay (IFA).RESULTS:
Serum samples (n=102) were tested by IgG-IFA and by ELISA. IFA and ELISA showed IgG-positive results in 77 and 73 samples, respectively. Qualitative concordance of 96% was demonstrated between the two techniques.CONCLUSIONS:
ELISA may be useful to diagnose HHV-7 infection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunoglobulin G
/
Herpesvirus 7, Human
/
Roseolovirus Infections
/
Antibodies, Viral
Type of study:
Diagnostic study
/
Prognostic study
/
Qualitative research
Limits:
Humans
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Journal subject:
Tropical Medicine
Year:
2020
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Hermínio Ometto/BR
/
Instituto Butantan/BR
/
Universidade Federal dos Vales do Jequinhonha e Mucuri/BR
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