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FOXC2-AS1 regulates phenotypic transition, proliferation and migration of human great saphenous vein smooth muscle cells
Zhang, Chuang; Li, Huixiang; Guo, Xueli.
  • Zhang, Chuang; Basic Medical College of Zhengzhou University. Department of Pathology. Zhengzhou. CN
  • Li, Huixiang; Basic Medical College of Zhengzhou University. Department of Pathology. Zhengzhou. CN
  • Guo, Xueli; The First Affiliated Hospital of Zhengzhou University. Department of Vascular Surgery. Zhengzhou. CN
Biol. Res ; 52: 59, 2019. graf
Article in English | LILACS | ID: biblio-1100911
ABSTRACT

OBJECTIVES:

In varicose veins, vascular smooth muscle cells (VSMCs) often shows phenotypic transition and abnormal proliferation and migration. Evidence suggests the FOXC2-Notch pathway may be involved in the pathogenesis of varicose veins. Here, this study aimed to explore the role of long non-coding RNA FOXC2-AS1 (FOXC2 antisense RNA 1) in phenotypic transition, proliferation, and migration of varicose vein-derived VSMCs and to explore whether the FOXC2-Notch pathway was involved in this process.

METHODS:

The effect of FOXC2-AS1 on the proliferation and migration of human great saphenous vein smooth muscle cells (SV-SMCs) was analyzed using MTT assay and Transwell migration assay, respectively. The levels of contractile marker SM22α and synthetic marker osteopontin were measured by immunohistochemistry and Western blot to assess the phenotypic transition.

RESULTS:

The human varicose veins showed thickened intima, media and adventitia layers, increased synthetic VSMCs, as well as upregulated FOXC2-AS1 and FOXC2 expression. In vitro assays showed that FOXC2-AS1 overexpression promoted phenotypic transition, proliferation, and migration of SV-SMCs. However, the effect of FOXC2-AS1 overexpression could be abrogated by both FOXC2 silencing and the Notch signaling inhibitor FLI-06. Furthermore, FOXC2-AS1 overexpression activated the Notch pathway by upregulating FOXC2.

CONCLUSION:

FOXC2-AS1 overexpression promotes phenotypic transition, proliferation, and migration of SV-SMCs, at least partially, by activating the FOXC2-Notch pathway.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Saphenous Vein / Cell Movement / Myocytes, Smooth Muscle / Cell Proliferation / Forkhead Transcription Factors Limits: Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Basic Medical College of Zhengzhou University/CN / The First Affiliated Hospital of Zhengzhou University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Saphenous Vein / Cell Movement / Myocytes, Smooth Muscle / Cell Proliferation / Forkhead Transcription Factors Limits: Humans Language: English Journal: Biol. Res Journal subject: Biology Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Basic Medical College of Zhengzhou University/CN / The First Affiliated Hospital of Zhengzhou University/CN