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Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition
Zhou, Yuan; Xue, Ruifeng; Wang, Jinglin; Ren, Haozhen.
  • Zhou, Yuan; Affiliated Drum Tower Hospital of Nanjing University Medical School. Department of Hepatobiliary Surgery. Nanjing. CN
  • Xue, Ruifeng; Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University. Department of Hepatobiliary Surgery. Nanjing. CN
  • Wang, Jinglin; Affiliated Drum Tower Hospital of Nanjing University Medical School. Department of Hepatobiliary Surgery. Nanjing. CN
  • Ren, Haozhen; Affiliated Drum Tower Hospital of Nanjing University Medical School. Department of Hepatobiliary Surgery. Nanjing. CN
Braz. j. med. biol. res ; 53(4): e8882, 2020. tab, graf
Article in English | LILACS | ID: biblio-1100927
ABSTRACT
Hepatocellular carcinoma (HCC) is one of the most common primary malignant tumors of the liver worldwide. Liver resection and transplantation are currently the only effective treatments; however, recurrence and metastasis rates are still high. Previous studies have shown that the epithelial-mesenchymal transition (EMT) is a key step in HCC invasion and metastasis. Inhibition of EMT has become a new therapeutic strategy for tumors. Recently, puerarin, a well-characterized component of traditional Chinese medicine, has been isolated from Pueraria radix and exerts positive effects on many diseases, particularly cancers. In this study, CCK-8, EdU immunofluorescence, colony formation, wound healing, and migration assays were used to detect the effects of puerarin on HCC cells. We further analyzed the relationship between puerarin and miR-21/PTEN/EMT markers in HCC cell lines. Our results showed that HCC cell proliferation, migration, invasion, tumor formation, and metastasis were reduced by puerarin in vitro and in vivo. Additionally, puerarin inhibited the EMT process of HCC by affecting the expression of Slug and Snail. Moreover, oncogenic miR-21 was inhibited by puerarin, coupled with an increase in the tumor suppressor gene PTEN. Increasing miR-21 expression or decreasing PTEN expression reversed the inhibition effects of puerarin in HCC. These data confirmed that puerarin affects HCC through the miR-21/PTEN/EMT regulatory axis. Overall, puerarin may represent a chemopreventive and/or chemotherapeutic agent for HCC treatment.
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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / PTEN Phosphohydrolase / Epithelial-Mesenchymal Transition / Isoflavones / Liver Neoplasms / Antineoplastic Agents Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Drum Tower Hospital of Nanjing University Medical School/CN / Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Carcinoma, Hepatocellular / PTEN Phosphohydrolase / Epithelial-Mesenchymal Transition / Isoflavones / Liver Neoplasms / Antineoplastic Agents Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2020 Type: Article Affiliation country: China Institution/Affiliation country: Affiliated Drum Tower Hospital of Nanjing University Medical School/CN / Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University/CN