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PSA secretion and cell proliferation are affected by NCoR1 silencing in prostate cancer cells
Costa, Bruna Pasqualotto; Brum, Ilma Simoni; Pizzolato, Lolita Schneider; Biolchi, Vanderlei; Branchini, Gisele.
  • Costa, Bruna Pasqualotto; Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA). Programa de Pós-Graduação em Patologia. Porto Alegre. BR
  • Brum, Ilma Simoni; Universidade Federal do Rio Grande do Sul (UFRGS). Instituto de Ciências Básicas da Saúde. Departamento de Fisiologia. Porto Alegre. BR
  • Pizzolato, Lolita Schneider; Universidade Federal do Rio Grande do Sul (UFRGS). Instituto de Ciências Básicas da Saúde. Departamento de Fisiologia. Porto Alegre. BR
  • Biolchi, Vanderlei; Universidade do Vale do Taquari (Univates). Centro de Ciências Biológicas e da Saúde. Lajeado. BR
  • Branchini, Gisele; Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA). Programa de Pós-Graduação em Patologia. Porto Alegre. BR
Clin. biomed. res ; 40(1): 37-43, 2020.
Article in English | LILACS | ID: biblio-1117078
ABSTRACT

Introduction:

The androgen receptor (AR) plays an important role in normal development of the prostate gland, as well as in prostatic neoplasms. Transcriptional regulation by AR is modulated by its interaction with co-activators or co-repressors, such as NCoR1 (nuclear receptor co-repressor 1), which is involved in reducing AR activity over the target gene transcription.

Methods:

To identify the role of NCoR1 in the prostate cancer androgen independence in a cell line model, we aimed to evaluate the effects of silencing NCoR1 on prostate-specific antigen (PSA) gene expression, the proliferative response and PSA secretion on the supernatant of C4-2B and LNCaP cells that were submitted to small interfering RNAs (siRNAs) transfection, and to treatments with different androgen dosages.

Results:

In LNCaP and C4-2B cells with no dihydrotestosterone (DHT) treatment, a decrease in PSA mRNA expression was observed 48 hours and 72 hours after gene silencing in the siNCoR group when compared to the control and siNC groups. The LNCaP and C4-2B cells showed a biphasic pattern in response to dihydrotestosterone treatment in transfected groups (siNCoR and siNC) as well as in the control condition (without transfection). The secretion of PSA in cell supernatant of LNCaP and C4-2B cells was higher in the siNCoR group, and, in relation to hormonal treatment, higher in the 10-8 M DHT group.

Conclusions:

A reduction in the NCoR1 levels seems to have a double influence on the activity of AR in PCa cells. These results suggest that NCoR may act as an AR co-repressor depending upon hormonal stimulation.(AU)
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Prostatic Neoplasms / Prostate-Specific Antigen / Cell Proliferation / Nuclear Receptor Co-Repressor 1 Type of study: Prognostic study Limits: Humans / Male Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)/BR / Universidade Federal do Rio Grande do Sul (UFRGS)/BR / Universidade do Vale do Taquari (Univates)/BR

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Full text: Available Index: LILACS (Americas) Main subject: Prostatic Neoplasms / Prostate-Specific Antigen / Cell Proliferation / Nuclear Receptor Co-Repressor 1 Type of study: Prognostic study Limits: Humans / Male Language: English Journal: Clin. biomed. res Journal subject: Medicine Year: 2020 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)/BR / Universidade Federal do Rio Grande do Sul (UFRGS)/BR / Universidade do Vale do Taquari (Univates)/BR