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A Thermotolerant Xylan-Degrading Enzyme Is Produced by Streptomyces malaysiensis AMT-3 Using by-Products From the Food Industry
Nascimento, Rodrigo Pires do; Reis, Alberto Delgado; Gírio, Francisco; Pereira Jr, Nei; Bon, Elba Pinto da Silva; Coelho, Rosalie Reed Rodrigues.
  • Nascimento, Rodrigo Pires do; Federal University of Rio de Janeiro. School of Chemistry. Center of Technology. Rio de Janeiro. BR
  • Reis, Alberto Delgado; National Laboratory of Energy and Geology. Lisbon. PT
  • Gírio, Francisco; National Laboratory of Energy and Geology. Lisbon. PT
  • Pereira Jr, Nei; Federal University of Rio de Janeiro. School of Chemistry. Center of Technology. Rio de Janeiro. BR
  • Bon, Elba Pinto da Silva; Federal University of Rio de Janeiro. Center of Nature and Mathematics Science. Biochemistry Institute. Rio de Janeiro. BR
  • Coelho, Rosalie Reed Rodrigues; Federal University of Rio de Janeiro. Center of Health Science. Microbiology Institute. Rio de Janeiro. BR
Braz. arch. biol. technol ; 63: e20190243, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132163
ABSTRACT
Abstract This study evaluated the production of endoxylanases by Streptomyces malaysiensis AMT-3 in submerged fermentation using by-products of the food industry at 28ºC. In shake-flasks experiments, the highest endoxylanase activity of 45.8 U.mL-1 was observed within 6 days in a medium containing (w/v) 2.5% wheat bran and 1.2% corn steep liquor. The same culture conditions were used to evaluate the enzyme production in a 2 L stirred tank reactor under different agitation (300, 450 and 600 rev.min-1) and aeration (30 and 60 L.h-1) conditions. The use of 450 rev.min-1 coupled to an aeration of 90 L.h-1 resulted on 81.3 U.mL-1 endoxylanase activity within 5 days. The effect of temperature and pH on endoxylanase activity and stability showed the highest activity at 60 ºC and pH 6.0. Zymography showed the presence of three xylanolytic bands with molecular masses of 690, 180 and 142 kDa. The results showed that the thermotolerant actinobacterial endoxylanase can be produced in high titers using by-product of the food industry.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Streptomyces / Temperature / Food Industry / Endo-1,4-beta Xylanases / Fermentation Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil / Portugal Institution/Affiliation country: Federal University of Rio de Janeiro/BR / National Laboratory of Energy and Geology/PT

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Full text: Available Index: LILACS (Americas) Main subject: Streptomyces / Temperature / Food Industry / Endo-1,4-beta Xylanases / Fermentation Language: English Journal: Braz. arch. biol. technol Journal subject: Biology Year: 2020 Type: Article Affiliation country: Brazil / Portugal Institution/Affiliation country: Federal University of Rio de Janeiro/BR / National Laboratory of Energy and Geology/PT