Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting
Rev. Soc. Bras. Med. Trop
;
53: e20200211, 2020. tab, graf
Article
in English
| SES-SP, ColecionaSUS, LILACS
| ID: biblio-1136883
ABSTRACT
Abstract INTRODUCTION:
Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M. tuberculosis complex and NTM species, and the characterization of NTM species is important. This study aimed to evaluate the performance of a molecular system based on multiplex real-time PCR with high-resolution melting (HRM) for the identification and differentiation of NTM species of clinical importance of an endemic area for tuberculosis in northeastern Brazil.METHODS:
The technical protocol of the molecular system was based on multiplex real-time PCR-HRM, and evaluated the sensitivity and specificity of the detection of NTM species in mycobacterial clinical isolates from the studied region. The gold standard method was specific gene sequencing.RESULTS:
The sensitivity and specificity of multiplex real-time PCR-HRM modified for differentiation between NTM and M. tuberculosis were 90% and 100%, respectively. The PCR-HRM sensitivities for the characterization of NTM species (M. kansasii, M. abscesses, M. avium, and M. fortuitum) were 94.59%, 80%, 57.14%, and 54%, respectively. CONCLUSIONS The multiplex real-time PCR-HRM modified assay has the potential to rapidly and efficiently identify nontuberculous mycobacteria of clinical importance, which is crucial for immediate implementation of the appropriate therapy and thus avoiding complications and sequelae in patients.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis
/
Mycobacterium Infections, Nontuberculous
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
/
Practice guideline
/
Prognostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Soc. Bras. Med. Trop
Year:
2020
Type:
Article
Institution/Affiliation country:
Centro de Referência Professor Hélio Fraga/BR
/
Fundação Oswaldo Cruz/BR
/
Laboratório Central de Saúde Pública Dr. Milton Bezerra Sobral/BR
/
Universidade de Pernambuco/BR
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