Differential proteomic analysis of children infected with respiratory syncytial virus
Braz. j. med. biol. res
;
54(4): e9850, 2021. tab, graf
Article
in English
| LILACS
| ID: biblio-1153545
ABSTRACT
Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Respiratory Syncytial Virus, Human
/
Respiratory Syncytial Virus Infections
Type of study:
Prognostic study
Limits:
Child
/
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2021
Type:
Article
Affiliation country:
China
Institution/Affiliation country:
College of Computer Science, Guangdong Polytechnic Normal University/CN
/
Guangdong Food and Drug Vocational College/CN
/
Guangzhou Medical University/CN
/
Nanfang Hospital, Southern Medical University/CN
/
Pediatric Research Institute, Qilu Childrens Hospital of Shandong University/CN
/
Shenzhen Childrens Hospital/CN
/
the First Affiliated Hospital, Jinan University/CN
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