Evaluation of HIF-1α and VEGF-A expression in radiation-induced cystitis: A case-control study
Int. braz. j. urol
;
47(2): 295-305, Mar.-Apr. 2021. tab, graf
Article
in English
| LILACS
| ID: biblio-1154442
ABSTRACT
ABSTRACT The standard treatment for locally advanced cervical cancer (CC) is chemoradiotherapy. Once the bladder receives part of the radiation, a typical inflammatory condition that configures radiation-induced cystitis may develop. Chronic radiation-induced cystitis is commonly characterized by the bladder new submucosal vascularization, which is typically fragile and favors hematuria. The current study aims to investigate if Hypoxia-Induced Factor (HIF-1α) and its transcriptional target Vascular Endothelial Growth Factor A (VEGF-A) could be a primary pathway leading to increased submucosal vascularization. HIF-1α and VEGF-A mRNA levels in bladder core biopsies from CC patients treated with radiotherapy versus untreated (non-irradiated) patients were analyzed using a droplet digital polymerase chain reaction technology. Gene expression results showed that HIF-1α and VEGF-A had no significant differences between bladder samples from patients previously irradiated and untreated patient samples. However, a direct relationship between the degree of late morbidity and the expression of HIF-1α and VEGF-A has been demonstrated. Despite the lack of statistical significance precludes a definitive conclusion, the data presented herein suggests that further studies investigating the role of HIF-1α in bladder neovascularization in radiation-induced cystitis are highly recommended.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Uterine Cervical Neoplasms
/
Cystitis
Type of study:
Observational study
Limits:
Female
/
Humans
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Nacional do Câncer/BR
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