Can platelet-rich plasma coating improve polypropylene mesh integration? An immunohistochemical analysis in rabbits
Int. braz. j. urol
;
47(2): 287-294, Mar.-Apr. 2021. tab, graf
Article
in English
| LILACS
| ID: biblio-1154463
ABSTRACT
ABSTRACT Purpose:
Despite high success rates in the treatment of urinary incontinence, complications related to the use of polypropylene (PP) meshes are still a concern, especially in vaginal prolapses surgeries. The objective of this study was to assess the effect of autologous platelet-rich plasma (PRP) coating on the integration of PP meshes implanted in the vaginal submucosa of rabbits. Materials andMethods:
Thirty adult New Zealand rabbits were randomly divided into two groups (n=15) PP, implanted with conventional PP meshes; and PRP, implanted with autologous PRP coated PP meshes. Animals in both groups (n=5) were euthanized at 7, 30 and 90 days postoperatively, the vaginas extracted and sent to immunohistochemical analysis for the assessment of the pro-inflammatory agent TNF-α, anti-inflammatory agents TGF-β and IL-13, collagen metabolism marker MMP-2, and angiogenesis marker CD-31. AxioVision™ image analysis was used for the calculation of the immunoreactive area and density. Statistical analysis was performed with ANOVA followed by Tukey test (p <0.05).Results:
Animals in the PRP group showed significantly increased expression of the angiogenesis agent CD-31 at all experimental times when compared to the PP group (p <0.0001). However, no differences concerning the expression of the other markers were observed between the groups.Conclusion:
The addition of autologous PRP gel to PP meshes can be simply and safely achieved and seems to have a positive effect on implantation site angiogenesis. Further investigations are required to ascertain PPR coated meshes clinical efficacy in prolapses and stress urinary incontinence surgeries.
Full text:
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Index:
LILACS (Americas)
Main subject:
Polypropylenes
/
Platelet-Rich Plasma
Limits:
Animals
Language:
English
Journal:
Int. braz. j. urol
Journal subject:
Urology
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Campinas - UNICAMP/BR
/
Universidade Estadual de Londrina - UEL/BR
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