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Local tissue reaction and histopathological characteristics of three different bulking agents: a rabbit model
Sabetkish, Shabnam; Mohseni, Mohammad Javad; Sabetkish, Nastaran; Kajbafzadeh, Abdol-Mohammad.
  • Sabetkish, Shabnam; Tehran University of Medical Sciences. Childrens Hospital Medical Center. Section of Tissue Engineering and Stem Cells Therapy. Tehran. IR
  • Mohseni, Mohammad Javad; Tehran University of Medical Sciences. Childrens Hospital Medical Center. Section of Tissue Engineering and Stem Cells Therapy. Tehran. IR
  • Sabetkish, Nastaran; Tehran University of Medical Sciences. Childrens Hospital Medical Center. Section of Tissue Engineering and Stem Cells Therapy. Tehran. IR
  • Kajbafzadeh, Abdol-Mohammad; Tehran University of Medical Sciences. Childrens Hospital Medical Center. Section of Tissue Engineering and Stem Cells Therapy. Tehran. IR
Int. braz. j. urol ; 47(2): 322-332, Mar.-Apr. 2021. tab, graf
Article in English | LILACS | ID: biblio-1154465
ABSTRACT
ABSTRACT

Purpose:

We assessed the efficacy and safety of a single injection of three bulking agents over the short- and long-term follow-ups in rabbits. Dermal and preputial matrices were compared with Deflux (DxHA) injection. Material and

methods:

Twenty-four rabbits were divided into three groups. Group I (n=8) underwent the injection of a lyophilized dermal matrix (LDM) beneath the seromuscular layer of the bladder wall. Rabbits in group II (n=8) were injected with lyophilized preputial matrix (LPM). Rabbits of group III (n=8) were injected with DxHA as the control group. They were followed up for 1 and 6 months after the injection. Subcutaneous injection of all bulking agents was also performed in nude mice. Biopsies were stained with LCA (leukocyte common antibody), CD68, CD31, and CD34. Scanning electron microscopy (SEM) and MTT assay were also performed.

Results:

Immunohistochemistry staining with CD68 and LCA revealed higher inflammation grade in LDM as compared with LPM and DxHA. Fibrosis grade was also higher in LDM both in short- and long-term follow-ups. However, no significant difference was detected in CD31 and CD34 staining between control and experimental groups. SEM analysis showed that the particle size of LPM was more similar to DxHA. MTT assay revealed that cell proliferation was similar in DxHA, LDM, and LPM. In-vivo assay in nude mice model showed more promising results in LPM as compared with LDM.

Conclusion:

The long-term results demonstrated that LPM was more similar to Deflux with the least local tissue reaction, inflammation, and fibrosis grade.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Dextrans / Hyaluronic Acid Type of study: Prognostic study Limits: Animals Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2021 Type: Article Affiliation country: Iran Institution/Affiliation country: Tehran University of Medical Sciences/IR

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Full text: Available Index: LILACS (Americas) Main subject: Dextrans / Hyaluronic Acid Type of study: Prognostic study Limits: Animals Language: English Journal: Int. braz. j. urol Journal subject: Urology Year: 2021 Type: Article Affiliation country: Iran Institution/Affiliation country: Tehran University of Medical Sciences/IR