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Effects of Nogo-A and its receptor on the repair of sciatic nerve injury in rats
Jiang, Junjie; Yu, Yuanchen; Zhang, Zhiwu; Ji, Yuan; Guo, Hong; Wang, Xiaohua; Yu, Shengjun.
  • Jiang, Junjie; Yantaishan Hospital. Department of Hand Surgery. Yantai. CN
  • Yu, Yuanchen; Yantaishan Hospital. Department of Clinical Laboratory. Yantai. CN
  • Zhang, Zhiwu; Yantaishan Hospital. Department of Hand Surgery. Yantai. CN
  • Ji, Yuan; Yantaishan Hospital. Department of Hand Surgery. Yantai. CN
  • Guo, Hong; Yantai City Municipal Government Hospital. Yantai. CN
  • Wang, Xiaohua; Yantaishan Hospital. Department of Clinical Laboratory. Yantai. CN
  • Yu, Shengjun; Yantaishan Hospital. Department of Hand Surgery. Yantai. CN
Braz. j. med. biol. res ; 54(9): e10842, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249339
ABSTRACT
Regeneration of injured peripheral nerves is an extremely complex process. Nogo-A (neurite outgrowth inhibitor-A) inhibits axonal regeneration by interacting with Nogo receptor in the myelin sheath of the central nervous system (CNS). The aim of this study was to investigate the effects of Nogo-A and its receptor on the repair of sciatic nerve injury in rats. Sprague-Dawley rats (n=96) were randomly divided into 4 groups control group (control), sciatic nerve transection group (model), immediate repair group (immediate repair), and delayed repair group (delayed repair). The rats were euthanized 1 week and 6 weeks after operation. The injured end tissues of the spinal cord and sciatic nerve were obtained. The protein expressions of Nogo-A and Nogo-66 receptor (NgR) were detected by immunohistochemistry. The protein expressions of Nogo-A, NgR, and Ras homolog family member A (RhoA) were detected by western blot. At 1 week after operation, the pathological changes in the immediate repaired group were less, and the protein expressions of Nogo-A, NgR, and RhoA in the spinal cord and sciatic nerve tissues were decreased (P<0.05) compared with the model group. After 6 weeks, the pathological changes in the immediate repair group and the delayed repair group were alleviated and the protein expressions decreased (P<0.05). The situation of the immediate repair group was better than that of the delayed repair group. Our data suggest that the expression of Nogo-A and its receptor increased after sciatic nerve injury, indicating that Nogo-A and its receptor play an inhibitory role in the repair process of sciatic nerve injury in rats.
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Full text: Available Index: LILACS (Americas) Main subject: Receptors, Cell Surface / Myelin Proteins Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Yantai City Municipal Government Hospital/CN / Yantaishan Hospital/CN

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Full text: Available Index: LILACS (Americas) Main subject: Receptors, Cell Surface / Myelin Proteins Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2021 Type: Article Affiliation country: China Institution/Affiliation country: Yantai City Municipal Government Hospital/CN / Yantaishan Hospital/CN