Host defense peptides clavanins A and MO reduce in vitro osteoclastogenesis
Braz. j. oral sci
;
20: e211512, jan.-dez. 2021. ilus
Article
in English
| BBO, LILACS
| ID: biblio-1254424
ABSTRACT
Aim:
Several systemic diseases, such as periodontitis and apical periodontitis, can cause extensive bone resorption. Host defense peptides may have the potential for the development of novel therapies for the bone resorption process. This study evaluated the potential of host defense peptides clavanins A, MO, and LL-37 in in vitro osteoclastogenesis.Methods:
RAW 264.7 cultures were stimulated with recombinant of receptor activator of nuclear factor kappa B ligand in the presence of different tested concentrations of host defense peptides, besides calcium hydroxide and doxycycline. Cellular viability, nitric oxide production, and a number of differentiated osteoclast-like cells were also evaluated.Results:
Results showed that none of the substances were cytotoxic, except for 128 µg.mL-1 of doxycycline after 3 days. Host defense peptides, calcium hydroxide, and doxycycline did not interfere in nitric oxide production or downregulated it. An exception was observed in the presence of 2 µg.mL-1 of doxycycline, in which nitric oxide production was up-regulated. All host defense peptides were capable of reducing osteoclast-like cell differentiation.Conclusion:
Host defense peptides clavanins A and MO demonstrated to be potential suppressors of osteoclastogenesis in vitro without interfering in cellular viability and nitric oxide production. These promising results need to be further analyzed in in vivo models of bone resorption
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Osteogenesis
/
Bone Resorption
/
Antimicrobial Cationic Peptides
/
Nitric Oxide
Type of study:
Prognostic study
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2021
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Catholic University of Brasilia/BR
/
Federal University of Mato Grosso do Sul/BR
/
University of Brasília/BR
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