Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil
Electron. j. biotechnol
;
52: 21-29, July. 2021. ilus, tab, graf
Article
in English
| LILACS
| ID: biblio-1283484
ABSTRACT
BACKGROUND:
Super-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.RESULTS:
In this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEXSPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FUSPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and 45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.CONCLUSIONS:
The findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Carcinoma, Hepatocellular
/
Fluorouracil
/
Liver Neoplasms
Limits:
Humans
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2021
Type:
Article
Affiliation country:
Iran
/
Iraq
Institution/Affiliation country:
Al-Qasim Green University/IQ
/
Al_kindy Medicine College/IQ
/
Hamadan University of Medical Science/IR
/
Iraq Ministry of Health/IQ
/
Mustansiriyah University/IQ
/
University of Misan/IQ
/
University of Technology/IQ
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