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Ameliorative effects of morel mushroom (Morchella esculenta) against Cadmium-induced reproductive toxicity in adult male rats / Efeitos benéficos do cogumelo morel (Morchella esculenta) contra a toxicidade reprodutiva induzida por cádmio em ratos machos adultos
Iqbal, T; Jahan, S; Ain, Q Ul; Ullah, H; li, C; Chen, L; Zhou, X.
  • Iqbal, T; Jilin University. College of Animal Science and Veterinary Medicine. Lab of Animal Genetics, Breeding and Reproduction. Changchun. CN
  • Jahan, S; Quaid-i-Azam University. Department of Animal Sciences. Reproductive Physiology Lab. Islamabad. PK
  • Ain, Q Ul; Quaid-i-Azam University. Department of Animal Sciences. Reproductive Physiology Lab. Islamabad. PK
  • Ullah, H; Quaid-i-Azam University. Department of Animal Sciences. Reproductive Physiology Lab. Islamabad. PK
  • li, C; Jilin University. College of Animal Science and Veterinary Medicine. Lab of Animal Genetics, Breeding and Reproduction. Changchun. CN
  • Chen, L; Jilin University. College of Animal Science and Veterinary Medicine. Lab of Animal Genetics, Breeding and Reproduction. Changchun. CN
  • Zhou, X; Jilin University. College of Animal Science and Veterinary Medicine. Lab of Animal Genetics, Breeding and Reproduction. Changchun. CN
Braz. j. biol ; 82: e250865, 2022. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1285604
ABSTRACT
Cadmium (Cd) is one of the major toxicants, which affects human health through occupational and environmental exposure. In the current study, we evaluated the protective effects of morel mushrooms against Cd-induced reproductive damages in rats. For this purpose, 30 male rats were divided into 6 groups (n=5/group), the first group served as the control group, second group was treated with an intraperitoneal (i.p) injection of 1 mg/kg/day of Cd. Third and fourth groups were co-treated with 1 mg/kg/day of Cd (i.p) and 10 and 20 mg/kg/day of morel mushroom extract (orally) respectively. The final 2 groups received oral gavage of 10 and 20 mg/kg/day of morel mushroom extract alone. After treatment for 17 days, the animals were euthanized, and testes and epididymis were dissected out. One testis and epididymis of each animal were processed for histology, while the other testis and epididymis were used for daily sperm production (DSP) and comet assay. Our results showed that Cd and morel mushrooms have no effect on animal weight, but Cd significantly decreases the DSP count and damages the heritable DNA which is reversed in co-treatment groups. Similarly, the histopathological results of tests and epididymis show that morel mushrooms control the damage to these tissues. Whereas the morel mushroom extract alone could enhance the production of testosterone. These results conclude that morel mushrooms not only control the damage done by Cd, but it could also be used as a protection mechanism for heritable DNA damage.
RESUMO
O cádmio (Cd) é um dos principais tóxicos, que afeta a saúde humana por meio da exposição ocupacional e ambiental. No presente estudo, avaliamos os efeitos protetores dos cogumelos morel contra os danos reprodutivos induzidos pelo Cd em ratos. Para tanto, 30 ratos machos foram divididos em 6 grupos (n = 5 / grupo); o primeiro grupo serviu de controle, o segundo grupo foi tratado com injeção intraperitoneal (i.p) de 1 mg / kg / dia de Cd. O terceiro e o quarto grupos foram cotratados com 1 mg / kg / dia de Cd (i.p) e 10 e 20 mg / kg / dia de extrato de cogumelo morel (por via oral), respectivamente. Os dois grupos finais receberam gavagem oral de 10 e 20 mg / kg / dia de extrato de cogumelo morel sozinho. Após o tratamento por 17 dias, os animais foram sacrificados e os testículos e o epidídimo foram dissecados. Um testículo e epidídimo de cada animal foram processados para histologia, enquanto o outro testículo e epidídimo foram usados para produção diária de esperma (DSP) e ensaio cometa. Nossos resultados mostraram que os cogumelos Cd e morel não têm efeito sobre o peso do animal, mas o Cd diminui significativamente a contagem de DSP e danifica o DNA hereditário, que é revertido em grupos de cotratamento. Da mesma forma, os resultados histopatológicos dos testículos e do epidídimo mostram que os cogumelos morel controlam os danos a esses tecidos. Considerando que o extrato de cogumelo morel sozinho pode aumentar a produção de testosterona. Esses resultados concluem que os cogumelos morel não apenas controlam os danos causados pelo Cd, mas também podem ser usados como um mecanismo de proteção para danos hereditários ao DNA.

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Full text: Available Index: LILACS (Americas) Main subject: Cadmium / Agaricales Limits: Animals Language: English Journal: Braz. j. biol Year: 2022 Type: Article Institution/Affiliation country: Jilin University/CN / Quaid-i-Azam University/PK

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Full text: Available Index: LILACS (Americas) Main subject: Cadmium / Agaricales Limits: Animals Language: English Journal: Braz. j. biol Year: 2022 Type: Article Institution/Affiliation country: Jilin University/CN / Quaid-i-Azam University/PK