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The effect of paraoxonase 1 (PON1) gene polymorphisms T(-107)C and L55M and diet composition on serum PON1 activity in women
Zanini, Bianka Machado; Burkert, Leticia; Santos, Fabiola Goettem dos; Masternak, Michal M; Crespo-Ribeiro, José Augusto; Barros, Carlos Castilho; Valle, Sandra Costa; Schneider, Augusto.
  • Zanini, Bianka Machado; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
  • Burkert, Leticia; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
  • Santos, Fabiola Goettem dos; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
  • Masternak, Michal M; University of Central Florida. Burnett School of Biomedical Sciences. College of Medicine. Orlando. US
  • Crespo-Ribeiro, José Augusto; Universidade Federal de Pelotas. Faculdade de Medicina. Departamento de Ginecologia. Pelotas. BR
  • Barros, Carlos Castilho; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
  • Valle, Sandra Costa; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
  • Schneider, Augusto; Universidade Federal de Pelotas. Faculdade de Nutrição. Departamento de Nutrição. Pelotas. BR
Arch. endocrinol. metab. (Online) ; 65(6): 787-793, Nov.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1349989
ABSTRACT
ABSTRACT

Objective:

The aim of this study was to evaluate the serum activity of PON1 in women according to SNPs L55M and T-107C and diet composition. Materials and

methods:

Blood and serum samples from 26 women were used. DNA extraction, PCR and digestion with restriction enzymes of the PCR fragment were performed for genotyping the PON1 SNPs T-107C and L55M. Serum PON1 activity was measured in a single time point. Patients completed the semi-quantitative food frequency questionnaire and diet composition was estimated.

Results:

Genotypic distribution for L55M SNP was 56% for the LL genotype, 32% for LM and 12% for MM; for the PON1 C(-107)T SNP it was 28% for the TT genotype, 41% for CT and 31% for CC. Individuals with C and L alleles had higher serum PON1 activity. Combining the two SNPs, we observed that individuals carrying the LL and CC genotypes had twice the activity of carriers of the TT and MM genotypes. Considering food intake, no significant difference was observed between genotypes and intake levels.

Conclusion:

PON1 T(-107)C and L55M SNPs exert a strong effect on serum PON1 activity in an additive manner and are more important than diet to predict serum PON1 activity.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Single Nucleotide / Aryldialkylphosphatase Language: English Journal: Arch. endocrinol. metab. (Online) Journal subject: Endocrinology / Metabolism Year: 2021 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Federal de Pelotas/BR / University of Central Florida/US

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Full text: Available Index: LILACS (Americas) Main subject: Polymorphism, Single Nucleotide / Aryldialkylphosphatase Language: English Journal: Arch. endocrinol. metab. (Online) Journal subject: Endocrinology / Metabolism Year: 2021 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Federal de Pelotas/BR / University of Central Florida/US