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Molecular characterization of Aeromonas hydrophila isolates from diseased fishes in district Kasur, Punjab, Pakistan / Caracterização molecular de isolados de Aeromonas hydrophila de peixes doentes no distrito de Kasur, Punjab, Paquistão
Tooba, L; Shahzad, A; Zahid, M; Muhammad, R; Anam, I; Abdur, R A; Mohammed, A A; Mater, H M.
  • Tooba, L; University of Veterinary and Animal Sciences. Department of Wildlife & Ecology. Discipline of Zoology. Lahore. PK
  • Shahzad, A; University of Veterinary and Animal Sciences. Department of Wildlife & Ecology. Discipline of Zoology. Lahore. PK
  • Zahid, M; University of Azad Jammu and Kashmir. Department of Biotechnology. Muzaffarabad. PK
  • Muhammad, R; University of Veterinary and Animal Sciences. Department of Wildlife & Ecology. Discipline of Zoology. Lahore. PK
  • Anam, I; University of Veterinary and Animal Sciences. Department of Biological Sciences. Lahore. PK
  • Abdur, R A; The University of Lahore. Department of Zoology. Sargodha. PK
  • Mohammed, A A; Najran University. Faculty of Applied Medical Sciences. Department of Clinical Laboratory Sciences. Najran. SA
  • Mater, H M; Najran University. Pharmacy School. Department of Pharmaceutical Chemistry. Najran. SA
Braz. j. biol ; 84: e254816, 2024. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1355894
ABSTRACT
Abstract Pakistan is an agricultural country and fisheries play a very important role in the economic development of the country. Different diseases are prevalent in Pakistani fish but information related to the causative agents is not well-known. Keeping in view the significance of bacterial pathogens as the causative agents of multiple fish diseases, the present study was conducted for identification, characterization and analysis of virulence genes of Aeromonas spp. isolated from diseased fishes. A total of fifty fish samples having multiple clinical indications were collected from different fish farms of district Kasur, Punjab Pakistan. For isolation of Aeromonas spp. samples were enriched and inoculated on Aeromonas isolation medium. Isolates were identified and characterized by different biochemical tests, Analytical Profile Index (API) 20E kit and Polymerase Chain Reaction (PCR) assays. All isolates were screened for three putative virulence genes including aerolysin (aer), haemolysin (hyl) and heat labile cytotonic enterotoxin (alt). Seven isolates of Aeromonas (A.) hydrophila were retrieved and identified based on API 20E. These isolates were further confirmed as A. hydrophila on the basis of PCR assays. Three isolates were detected positive for the presence of virulence genes (alt and hyl). Whereas aerolysin (aer) gene was not present in any of A. hydrophila isolates. The present study confirmed A. hydrophila as the causative agent of epizootic ulcerative syndrome and motile Aeromonas septicemia in fish farms of district Kasur, Punjab Pakistan. Moreover, detection of two virulence genes (alt and hyl) in A. hydrophila isolates is a threat for fish consumers of study area.
RESUMO
Resumo O Paquistão é um país agrícola, onde a pesca desempenha um papel muito importante para o desenvolvimento econômico. Diferentes doenças são prevalentes em peixes do Paquistão, mas as informações relacionadas aos agentes causadores não são bem conhecidas. Tendo em vista a importância dos patógenos bacterianos como agentes causadores de múltiplas doenças em peixes, o presente estudo foi conduzido para identificação, caracterização e análise de genes de virulência de isolados de Aeromonas spp. de peixes doentes. Foram coletadas 50 amostras de peixes com múltiplas indicações clínicas em diferentes fazendas do distrito de Kasur, Punjab, Paquistão. Para isolar Aeromonas spp., as amostras foram enriquecidas e inoculadas em meio de isolamento. Os isolados foram identificados e caracterizados por diferentes testes bioquímicos, kit Analytical Profile Index (API) 20E, e ensaios de reação em cadeia da polimerase (PCR). Todos os isolados foram selecionados para três genes de virulência putativos, incluindo aerolisina (aer), hemolisina (hyl) e enterotoxina citotônica termolábil (alt). Sete isolados de Aeromonas hydrophila foram recuperados e identificados com base no API 20E. Esses isolados foram posteriormente confirmados como A. hydrophila de acordo com ensaios de PCR. Três isolados indicaram a presença de genes de virulência (alt e hyl), enquanto o gene aerolisina (aer) não esteve presente em nenhum dos isolados de A. hydrophila. O presente estudo confirmou A. hydrophila como o agente causador da síndrome ulcerativa epizoótica e septicemia móvel por Aeromonas em fazendas de peixes, no distrito de Kasur, Punjab, Paquistão. Além disso, a detecção de dois genes de virulência (alt e hyl) em isolados de A. hydrophila é uma ameaça para os consumidores de peixes da área de estudo.

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Full text: Available Index: LILACS (Americas) Main subject: Gram-Negative Bacterial Infections / Aeromonas Limits: Animals Country/Region as subject: Asia Language: English Journal: Braz. j. biol Year: 2024 Type: Article Institution/Affiliation country: Najran University/SA / The University of Lahore/PK / University of Azad Jammu and Kashmir/PK / University of Veterinary and Animal Sciences/PK

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Full text: Available Index: LILACS (Americas) Main subject: Gram-Negative Bacterial Infections / Aeromonas Limits: Animals Country/Region as subject: Asia Language: English Journal: Braz. j. biol Year: 2024 Type: Article Institution/Affiliation country: Najran University/SA / The University of Lahore/PK / University of Azad Jammu and Kashmir/PK / University of Veterinary and Animal Sciences/PK