Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy
Acta cir. bras
;
36(11): e361104, 2021. tab, graf
Article
in English
| LILACS, VETINDEX
| ID: biblio-1360061
ABSTRACT
ABSTRACT Purpose:
To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models.Methods:
The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis.Results:
Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein.Conclusions:
Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Hepatic Veno-Occlusive Disease
/
Drugs, Chinese Herbal
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Year:
2021
Type:
Article
Institution/Affiliation country:
Faculty of Pharmacy/CN
/
Ningbo University/CN
/
Ningbo Yinzhou No.2 Hospital/CN
/
Wenzhou Medical University/CN
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