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Paeoniflorin mitigates PBC-induced liver fibrosis by repressing NLRP3 formation
Zhang, Yizhe; Zhang, Shujie; Luo, Xin; Zhao, Han; Xiang, Xiaoxing.
  • Zhang, Yizhe; Zhengzhou University. College of life Science. Zhongyuan District. CN
  • Zhang, Shujie; Zhengzhou University. College of life Science. Zhongyuan District. CN
  • Luo, Xin; Zhengzhou University. College of life Science. Zhongyuan District. CN
  • Zhao, Han; Zhengzhou University. College of life Science. Zhongyuan District. CN
  • Xiang, Xiaoxing; Taizhou peoples Hospital affiliated to Medical College of Yangzhou University. Department of Gastroenterology. Jiangsu. CN
Acta cir. bras ; 36(11): e361106, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1360062
ABSTRACT
ABSTRACT

Purpose:

To delve into the influence of paeoniflorin (PA) on abating primary biliary cholangitis (PBC)-induced liver fibrosis and its causative role.

Methods:

Our team allocated the mice to control group, PA group, PBC group and PBC+PA group. We recorded the weight change of mice in each group. We used Masson staining for determining liver fibrosis, immunofluorescence staining for measuring tumor necrosis factor-α (TNF-α) expression, quantitative real-time polymerase chain reaction (qRT-PCR) for assaying related gene expression, as well as Western blot for testing related protein expression.

Results:

The weight of PBC model mice declined. Twenty-four weeks after modeling, the positive rate of anti-mitochondrial antibody-M2 (AMA-M2) in PBC mice reached 100%. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), hydroxyproline (HYP), laminin (LN), procollagen type III (PC III), and malondialdehyde (MDA) contents saliently waxed (p<0.01). Meanwhile, superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) activity patently waned (p<0.01). Liver fibrosis levels were flagrantly higher (p<0.01), and TNF-α, NOD-like receptor protein 3 (NLRP3), caspase-1, interleukin-18 (IL-18), and interleukin-1β (IL-1β) protein or gene expression were manifestly up-regulated (p<0.01). PA could restore the weight of PBC mice, strikingly restrain the positive expression of AMA-M2, and down-regulate serum ALP, ALT, AST, HYP, LN, PC III, MDA in PBC mice (p<0.01). PA could also significantly up-regulate SOD and GSH-px levels (p<0.01), down-regulate IL-1β, IL-18, caspase-1, NLRP3, and TNF-α protein or gene expression in PBC mice (p<0.01) and inhibit liver fibrosis levels (p<0.01).

Conclusions:

PA can reduce PBC-induced liver fibrosis in mice and may function by curbing the formation of NLRP3.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Monoterpenes / NLR Family, Pyrin Domain-Containing 3 Protein / Glucosides / Liver Cirrhosis Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Year: 2021 Type: Article Institution/Affiliation country: Taizhou peoples Hospital affiliated to Medical College of Yangzhou University/CN / Zhengzhou University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Monoterpenes / NLR Family, Pyrin Domain-Containing 3 Protein / Glucosides / Liver Cirrhosis Type of study: Prognostic study Limits: Animals Language: English Journal: Acta cir. bras Year: 2021 Type: Article Institution/Affiliation country: Taizhou peoples Hospital affiliated to Medical College of Yangzhou University/CN / Zhengzhou University/CN