The possibility of early diagnosis of colorectal cancer through expression of Lncrna UCA1, Lncrna LINC00970, and Wnt genes; an in-vitro study
Acta sci., Health sci
;
44: e56960, Jan. 14, 2022.
Article
in English
| LILACS
| ID: biblio-1367539
ABSTRACT
Colorectal cancer is the 4thcause of cancer death; with considering the growth process of this cancer and the necessity of early diagnosis, the purpose of the research is to state the LncRNA 00970, LncRNA UCAI,and the Wntgene before and after the treatment by 5-Azacytidine epigenetic medicine, to reach the biomarker in the very first steps of colorectal cancer. In this experiment, the human colon cancer cell line (HT29) treated with different concentrations of 5-aza-2'-deoxycytidine (5-aza-dC) was utilized to induce DNA demethylation; Quantitative PCR (qPCR) was used to measure LncRNA UCA1and LncRNA LINC00970 and Wntexpression. There was a significant relationship between the expression of LncRNA 00970, LncRNA UCAI,and the Wntgene and its effects on colorectal (p < 0.05). The Wntgene was treated by 1 and 10 of 5-Azacytidine epigenetic medicine, which then experienced decreases. In LncRNA UCAI and LncRNA00970 in dose 1 micromolar of 5-Azacytidine had decrement and increment of expressionrespectively that explains their efficiency but in treatment by dose 10 mM of this medicine, no significant LncRNA expression difference was detected, 5-azacitidine has a direct impact on its target genes and LncRNAs.Therefore, it can be used in the early diagnosis of colorectal cancer.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
DNA
/
Colorectal Neoplasms
/
Colonic Neoplasms
/
Early Diagnosis
Type of study:
Controlled clinical trial
/
Diagnostic study
/
Evaluation studies
/
Screening study
Language:
English
Journal:
Acta sci., Health sci
Journal subject:
Medicina
/
Sa£de P£blica
Year:
2022
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Islamic Azad University/IR
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