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Antimicrobial Effect and Surface Tension of Some Chelating Solutions with Added Surfactants
Giardino, Luciano; Andrade, Flaviana Bombarda de; Beltrami, Riccardo.
  • Giardino, Luciano; Scientist Researcher. Crotone. IT
  • Andrade, Flaviana Bombarda de; Scientist Researcher. Crotone. IT
  • Beltrami, Riccardo; Scientist Researcher. Crotone. IT
Braz. dent. j ; 27(5): 584-588, Sept.-Oct. 2016. tab, graf
Article in English | LILACS | ID: biblio-828042
ABSTRACT
Abstract This study assessed the antimicrobial efficacy and surface tension of established irrigating solutions with a new experimental chelating solution in infected dentin tubes. Twenty-five specimens were randomly assigned to each of the irrigating solutions. Twenty specimens were used as negative and positive controls. After 21 days of contamination with E. faecalis, the irrigating solutions MTAD, QMiX and Tetraclean NA were delivered into each infected root canal. The solutions were removed and dentin samples were withdrawn from the root canals with sterile low-speed round burs with increasing ISO diameters. The dentin powder samples obtained with each bur were immediately collected in separate test tubes containing 3 mL of BHI broth. After that, 100 μL from each test tube was cultured on blood agar. The grown colonies were counted and recorded as colony-forming units (CFU). The surface tension of the irrigants was measured using a Cahn DCA-322 Dynamic Contact Angle Analyzer. A Kruskal Wallis nonparametric ANOVA and a Friedman test were used (p<0.05). Tetraclean NA showed lower surface tension and CFU values than MTAD and QMiX. Better antibacterial action and low surface tension were observed for Tetraclean NA, probably due to the improved penetration into the root canal and dentinal tubes
RESUMO
Resumo Este estudo avaliou a eficácia antimicrobiana e tensão superficial de soluções irrigadoras e uma nova solução quelante em tubos de dentina infectada. Vinte e cinco espécimes foram aleatoriamente distribuídos conforme as soluções irrigantes. Decorrifdos 21 dias de contaminação com E. faecalis, a soluções de irrigação MTAD, QMiX e Tetraclean NA foram distribuídas em cada canal radicular infectado. As soluções foram removidas e as amostras de dentina foram retiradas dos canais radiculares com brocas esféricas de baixa velocidade com diâmetros ISO sucessivamente maiores. As amostras do de dentina obtidas com cada broca foram imediatamente colocadas em tubos de ensaio separados contendo 3 mL de caldo BHI. A seguir, 100 μL de cada amostra do tubo de teste foi cultivada em agar de sangue. As colônias crescidas foram contadas e registadas como unidades formadoras de colônias (UFC). A tensão superficial das soluções irrigantes foi medida utilizando o método de Wilhelmy. A análise não paramétrica de Kruskal-Wallis e o teste de Friedman foram utilizados (p<0,05). Tetraclean NA apresentou menor tensão de superfície e menores valores de UFC do que MTAD e QMiX. A melhor ação antibacteriana e baixa tensão superficial foram observadas para Tetraclean NA, provavelmente devido à melhor penetração no canal radicular e túbulos dentinários.
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Full text: Available Index: LILACS (Americas) Main subject: Surface-Active Agents / Surface Tension / Chelating Agents / Anti-Infective Agents Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Italy Institution/Affiliation country: Scientist Researcher/IT

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Full text: Available Index: LILACS (Americas) Main subject: Surface-Active Agents / Surface Tension / Chelating Agents / Anti-Infective Agents Limits: Animals Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2016 Type: Article Affiliation country: Italy Institution/Affiliation country: Scientist Researcher/IT