A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production
Braz. j. microbiol
;
48(3): 397-402, July-Sept. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-889149
ABSTRACT
Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Sugar Acids
/
Bacteriological Techniques
/
Rhodobacteraceae
Type of study:
Diagnostic study
/
Evaluation studies
/
Screening study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2017
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Institute of Applied Ecology/CN
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