Your browser doesn't support javascript.
loading
A novel expression vector for the secretion of abaecin in Bacillus subtilis
Li, Li; Mu, Lan; Wang, Xiaojuan; Yu, Jingfeng; Hu, Ruiping; Li, Zhen.
  • Li, Li; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
  • Mu, Lan; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
  • Wang, Xiaojuan; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
  • Yu, Jingfeng; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
  • Hu, Ruiping; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
  • Li, Zhen; College of Basic Medicine. Inner Mongolia Medical University. Hohhot. CN
Braz. j. microbiol ; 48(4): 809-814, Oct.-Dec. 2017. graf
Article in English | LILACS | ID: biblio-889176
ABSTRACT
ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Bacillus subtilis / Insect Proteins / Antimicrobial Cationic Peptides / Genetic Vectors Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: College of Basic Medicine/CN

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Bacillus subtilis / Insect Proteins / Antimicrobial Cationic Peptides / Genetic Vectors Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2017 Type: Article Affiliation country: China Institution/Affiliation country: College of Basic Medicine/CN