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One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil
Andrade, Elisabete; Rocha, Daniele; Fontana-Maurell, Marcela; Costa, Elaine; Ribeiro, Marisa; de Godoy, Daniela Tupy; Ferreira, Antonio G P; Tanuri, Amilcar; Alvarez, Patrícia; Brindeiro, Rodrigo.
  • Andrade, Elisabete; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Rocha, Daniele; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Fontana-Maurell, Marcela; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Costa, Elaine; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Ribeiro, Marisa; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • de Godoy, Daniela Tupy; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Ferreira, Antonio G P; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Tanuri, Amilcar; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
  • Alvarez, Patrícia; Fundação Oswaldo Cruz (Fiocruz). Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos. Rio de Janeiro. BR
  • Brindeiro, Rodrigo; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
Braz. j. infect. dis ; 22(5): 418-423, Sept.-Oct. 2018. tab, graf
Article in English | LILACS | ID: biblio-974233
ABSTRACT
ABSTRACT The Brazilian Public Health Service provides freely αPEG-IFN to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects the treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay and it has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit (Cobas/Taqman HCV Test v2.0 - Roche), showing virus load results with significant correlation between them (p= 0,012) using commercial and clinical panels. In addition, 611 samples from patients treated with peguilated alfa-interferon (αPEG-IFN) from different regions of Brazil were analyzed. Our one-step real-time RT-PCR assay demonstrated good performance in viral load measurement and in treatment course monitoring, with acceptable sensitivity and specificity values.
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Full text: Available Index: LILACS (Americas) Main subject: RNA, Viral / Hepatitis C / Hepacivirus / Viral Load / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz (Fiocruz)/BR / Universidade Federal do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: RNA, Viral / Hepatitis C / Hepacivirus / Viral Load / Real-Time Polymerase Chain Reaction Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2018 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Oswaldo Cruz (Fiocruz)/BR / Universidade Federal do Rio de Janeiro/BR