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The role of PI3K/Akt signal pathway in the protective effects of propofol on intestinal and lung injury induced by intestinal ischemia/reperfusion
Li, Qingwen; Cui, Shanshan; Jing, Guoqing; Ding, Huang; Xia, Zhongyuan; He, Xianghu.
  • Li, Qingwen; Wuhan University. Renmin Hospital. Department of Anesthesiology. Hubei. CN
  • Cui, Shanshan; Wuhan University. Renmin Hospital. Department of Anesthesiology. Hubei. CN
  • Jing, Guoqing; Wuhan University. Renmin Hospital. Department of Anesthesiology. Hubei. CN
  • Ding, Huang; Wuhan University. Renmin Hospital. Department of Anesthesiology. Hubei. CN
  • Xia, Zhongyuan; Wuhan University. Renmin Hospital. Department of Anesthesiology. Hubei. CN
  • He, Xianghu; Wuhan University. Zhongnan Hospital. Department of Anesthesiology. Hubei. CN
Acta cir. bras ; 34(1): e20190010000005, 2019. graf
Article in English | LILACS | ID: biblio-983682
ABSTRACT
Abstract

Purpose:

To investigate the role of PI3k/Akt signal pathway in the protective effects of propofol on intestinal and lung injury induced by intestinal ischemia/reperfusion(I/R).

Methods:

Male Sprague-Dawley rats were subjected to 45 min of ischemia by occluding the superior mesenteric artery and to 2h of reperfusion to establish the model of I/R. Twenty four rats were randomly divided into four groups Sham, intestinal I/R (II/R), propofol (P), wortmannin (W). In groups P, W, propofol was injected intravenously and continuously at the onset of reperfusion via infusion pump. PI3K inhibitor (wortmannin) was administered intravenously in group W 25 min before ischemia. Intestinal tissues and lung tissues were obtained for determination of histologic injury, wet/dry weight ratio, malondialdehyde (MDA) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. Meanwhile, the expressions of caspase-3 and phosphorylated Akt (p-Akt) in intestines and lungs were detected by western blot.

Results:

Propofol treatment alleviated intestinal and lung morphological changes which were observed in II/R group,Moreover, wet/dry weight ratio, the MDA level, MPO activity and expression of caspase-3 were significantly decreased whereas the SOD activity and p-Akt expression were significantly increased. Notably, the protections were significantly reversed by pretreatment of wortmannin.

Conclusion:

PI3K/Akt pathway activation play a critical role in the protective effects of propofol on intestinal and lung injury induced by ischemia/reperfusion.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Propofol / Anesthetics, Intravenous / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Lung Injury / Mesenteric Ischemia Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Wuhan University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Reperfusion Injury / Propofol / Anesthetics, Intravenous / Phosphatidylinositol 3-Kinases / Proto-Oncogene Proteins c-akt / Lung Injury / Mesenteric Ischemia Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Wuhan University/CN