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Protective effect of dexmedetomidine against organ dysfunction in a two-hit model of hemorrhage/resuscitation and endotoxemia in rats
Jiang, Yuanxu; Xia, Mingzhu; Huang, Qiang; Ding, Dengfeng; Li, Yali; Zhang, Zhongjun; Zhang, Xueping.
  • Jiang, Yuanxu; Jinan University. Department of Anesthesiology. Shenzhen. CN
  • Xia, Mingzhu; Luohu Hospital group. Hubei Community Health Service Center. Shenzhen. CN
  • Huang, Qiang; Jinan University. Department of Anesthesiology. Shenzhen. CN
  • Ding, Dengfeng; Jinan University. Department of Anesthesiology. Shenzhen. CN
  • Li, Yali; Jinan University. Department of Anesthesiology. Shenzhen. CN
  • Zhang, Zhongjun; Jinan University. Department of Anesthesiology. Shenzhen. CN
  • Zhang, Xueping; Jinan University. Department of Anesthesiology. Shenzhen. CN
Braz. j. med. biol. res ; 52(3): e7905, 2019. tab, graf
Article in English | LILACS | ID: biblio-984036
ABSTRACT
Dexmedetomidine (DEX), a selective agonist of α2-adrenergic receptors, has anti-inflammation properties and potential beneficial effects against trauma, shock, or infection. Therefore, this study aimed to investigate whether DEX might protect against multiple-organ dysfunction in a two-hit model of hemorrhage/resuscitation (HS) and subsequent endotoxemia. Eighty Wistar rats were randomized into four groups NS (normal saline), HS/L (HS plus lipopolysaccharide), HS/L+D (HS/L plus dexmedetomidine), and HS/L+D+Y (HS/L+D plus yohimbine). Six hours after resuscitation, blood gas (PaO2) and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urine nitrogen (BUN), creatinine (Cr), TNF-α, IL-β, IL-6, IL-8, IL-10, and nitric oxide (NO) were measured. The histopathology was assayed by staining. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels and heme oxygenase-1 (HO-1) were assayed. The PaO2 levels in HS/L rats were lower whereas the ALT, AST, BUN, Cr, TNF-α, IL-β, IL-6, IL-8, IL-10, and NO levels were higher compared to the control group. The HS/L+D increased PaO2 and further increased IL-10 and decreased ALT, AST, BUN, Cr, TNF-α, IL-β, IL-6, IL-8, and NO levels of the HS/L groups. In addition, the MDA in the HS/L groups increased whereas SOD activity decreased compared to the control group. Moreover, the HO-1 expression levels were increased by DEX administration in lung, liver, and kidney tissues. Lungs, livers, and kidneys of the HS/L group displayed significant damage, but such damage was attenuated in the HS/L+D group. All of the above-mentioned effects of DEX were partly reversed by yohimbine. DEX reduced multiple organ injury caused by HS/L in rats, which may be mediated, at least in part, by α2-adrenergic receptors.
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Full text: Available Index: LILACS (Americas) Main subject: Resuscitation / Endotoxemia / Protective Agents / Dexmedetomidine / Hemorrhage / Multiple Organ Failure Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Jinan University/CN / Luohu Hospital group/CN

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Full text: Available Index: LILACS (Americas) Main subject: Resuscitation / Endotoxemia / Protective Agents / Dexmedetomidine / Hemorrhage / Multiple Organ Failure Type of study: Prognostic study Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Jinan University/CN / Luohu Hospital group/CN