Expression of alpha4, alphav, beta1 and beta3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes
IJRM-Iranian Journal of Reproductive Medicine. 2014; 12 (9): 623-632
in English
| IMEMR
| ID: emr-149664
ABSTRACT
It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes [PCOS]. In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model. 30 NMRI female mice were equally divided into 3 groups control, experimental [PCOS that was injected estradiol valerate [40 mg/kg]]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; alpha4, alpha v, beta 1 and beta 3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectively. Estradiol level was significantly increased [p = 0.035] in PCOS group. Based on our finding, the ratio of genes' expressions alphav, beta 3, beta 1 and alpha 4 in PCOS to control group was 0.479 +/- 0.01, 0.5 +/- 0.001, 2.7 +/- 0.4 and 1.023 +/- 0.2 respectively. Genes expression showed a great difference [p = 0.001] between beta 3, beta 1 and alphav in PCOS compared to other groups. alpha v and beta 3 integrin proteins expressed in all groups but intensity of these proteins in PCOS groups, was lower than other groups. Pattern of alpha v and beta3 integrins expression on the mouse blastocyst surface has an important effect during the implantation window. This pattern has changed in PCOS model and might have a great influence on implantation failure. Therefore, this experimental study suggests that a great attention to this problem may be essential in patients who are involved
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Embryo Implantation
/
Blastocyst
/
Integrins
/
Integrin beta1
/
Integrin alpha4
/
Integrin alphaV
/
Integrin beta3
/
Mice
Limits:
Animals
Language:
English
Journal:
Iran. J. Reprod. Med.
Year:
2014
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