Improvement of kidney apelin and apelin receptor in nitro-L-arginine-methyl ester induced rats

ABSTRACT

We have investigated the effect of 8 weeks aerobic training [AT] and Ferula gummosis supplement [FG] on apelin and apelin receptor [APJ], nitric oxide [NO] and angiotensin converting enzyme [ACE] of hypertensive rats. In a experimental study, 50 adult male wistar rats were classified into five groups; 1- AT, 2- FG, 3- combination of aerobic training + Ferula Gummosa supplement [TFG], 4- nitro-L-arginine-methyl ester [L-NAME], 5- shame [control] groups [SH]. The rats in the 1 to 4 groups received L-NAME [10 mg/kg, 6 times a week for 8 weeks]. Also, the 1 and 3 groups experienced the training of 15 to 22 m/min for 25 to 64 minutes, 5 times a week for 8 weeks, whereas, the 2 and 3 groups received Ferula gummosis supplement [90 mg/kg, 6 times a week for 8 weeks]. However, rats in 5 groups received NaCl solution. At protocols resulted in a significant increase in apelin and APJ as compared to control and L-NAME groups. The TFG protocols resulted in a markedly increase in apelin, APJ and significantly decrease of ACE levels as compared to L-NAME group. Chronically administration of L-NAME resulted increased, ACE, and reduced the levels of apelin, APJ and NO, as compared to control group. The results in this study show that physical regular activity with and without herbal treatment induce amplification in apelin/APJ system and down-regulation blood pressure in L-NAME induced hypertension in the rat kidney tissue