Optimization of porous silicon conditions for DNA-based biosensing via reflectometric interference spectroscopy

ABSTRACT

Objective: Substantial effort has been put into designing DNA-based biosensors, which are commonly used to detect presence of known sequences including the quantification of gene expression. Porous silicon [PSi], as a nanostructured base, has been commonly used in the fabrication of optimally transducing biosensors. Given that the function of any PSi-based biosensor is highly dependent on its nanomorphology, we systematically optimized a PSi biosensor based on reflectometric interference spectroscopy [RIS] detecting the high penetrance breast cancer susceptibility gene, BRCA1