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Improvement of the slide hemagglutination test for rapid Chagas' disease screening in epidemiological surveys
Braz. j. med. biol. res ; 29(5): 623-8, May 1996. tab, graf
Article in En | LILACS | ID: lil-182545
Responsible library: BR1.1
ABSTRACT
A slide hemagglutination test, here called SHAT, which is practical and economical for seroepidemiological surveys was standardized. This is an improved modification of the rapid hemagglutination test (RHA) which utilizes a short-lived reagent prepared with fresh blood cells. The reagent and conditions of the test were considerably modified and, most importantly, an alkaline-solubilized Trypanosoma cruzi epimastigote antigen reagent is proposed. The stability of the SHAT reagent was at least one year at 4 degrees Celsius, in an appropriate liquid suspension. The SHAT was applied to 71 serum samples from patients with Chagas' disease and from 235 clinically healthy blood donors. Sensitivity, specificity and positive and negative predictive values for the selected cutoff titer corresponding to 14 dilution were 0.972 (0.903-0.992)9 O.983 (O.957-0.993) 0.945 (O.867-0.979) and O.991 (O.969-0.998), respectively. These values were comparable to those found for the RHA, immunofluorescence (IFT), indirect hemagglutination (IHAT) and complement fixation (CFT) tests. These data suggest that the SHAT should be useful for seroepidemiological surveys conducted at public health laboratories in developing countries.
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Index: LILACS Main subject: Hemagglutination Tests / Chagas Disease Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Language: En Journal: Braz. j. med. biol. res Journal subject: BIOLOGIA / MEDICINA Year: 1996 Type: Article
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Index: LILACS Main subject: Hemagglutination Tests / Chagas Disease Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Humans Language: En Journal: Braz. j. med. biol. res Journal subject: BIOLOGIA / MEDICINA Year: 1996 Type: Article