Description of an in vivo model for assessment of eosinophil chemoattractants in the mouse
Mem. Inst. Oswaldo Cruz
;
92(supl.2): 211-4, Dec. 1997. tab, graf
Article
in English
| LILACS
| ID: lil-202035
ABSTRACT
Chemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration in vivo. Eosinophils were purified from the blood of mice over-expressing the IL-5 gene and labelled with 111In. Only the C-C chemokines, eotaxin and MIP-1 alpha, but not RANTES, MCP-1, MCP-3, MIP-1ß. KC and MIP-2, effectively induced the recruitment of 111In-eosinophils in mouse skin. We suggest that this mouse model will be useful in assessing the role of endogenously-generated chemokines in mediating eosinophil migration to sites of allergic inflammation in vivo.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Monocyte Chemoattractant Proteins
/
Chemokines
/
Eosinophils
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1997
Type:
Article
/
Congress and conference
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