In situ hybridization and immunoperoxidase for quick detection and identification of leishmania braziliensis in tissue
Parasitol. día
; 20(3/4): 86-90, jul.-dic. 1996. ilus, tab
Article
in En
| LILACS
| ID: lil-202465
Responsible library:
CL1.1
ABSTRACT
The combined used of in situ hybridization and immunoperoxidase techniques applied to histological sections of the biopsy allowed an increment of the efficiency in the detection of Leishmania diagnosis.The molecular basis for DNA diagnosis is to allow labelled single-stranded species or strain-specific DNA sequences, selected from well characterised reference species, to find and hybridize with homologous DNA from or in, the unknown isolates of parasites. The use of immunoperoxidase assay was useful in disclosing the antigen pathways and revealed amastigotes and antigenic material in various morphological patterns
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Index:
LILACS
Main subject:
Leishmania braziliensis
/
Immunoenzyme Techniques
/
In Situ Hybridization
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Parasitol. día
Journal subject:
PARASITOLOGIA
Year:
1996
Type:
Article