CENP-B is a conserved gene among vegetal species
Genet. mol. res. (Online)
; 1(3): 241-245, Sept. 2002. ilus
Article
in En
| LILACS
| ID: lil-357426
Responsible library:
BR1.1
ABSTRACT
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a complete human CENP-B gene. In all the plant species, anti-CENP-B antibodies recognized an 80-kDa protein. A 360-bp sequence encoding for the amino terminus region of the CENP-B protein was amplified by PCR in all the species and the nick translated pG/CNPB probe hybridized with the PCR products. Apparently the CENP-B centromere protein or an equivalent protein is widely distributed in the vegetal kingdom.
Full text:
1
Index:
LILACS
Main subject:
Vegetables
/
Chromosomal Proteins, Non-Histone
Limits:
Humans
Language:
En
Journal:
Genet. mol. res. (Online)
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2002
Type:
Article