Técnica de citometria de fluxo para avaliação da produção de espécies reativas de oxigênio pelas células do líquido sinovial de eqüinos / Cytometry flow techniques for evaluation of reactive oxygen species produced by equine synovial cells fluid

ABSTRACT

Using the cytometry flow analysis and adapting the method proposed by Hasui et al. (1985), it was possible to quantify the reactive oxygen species (ROE) produced by distinct cell populations of the synovial fluid from rigid and inflamed joints in horses, and to identify an increase in the polymorphonuclear cells population densities in the inflamed joints. These data strengthen the premise that cytometry flow techniques can contribute for future researches on the articular cartilage degeneration in horses.