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Transcription of meiotic-like-pathway genes in Giardia intestinalis
Melo, Sandra P; Gómez, Vanessa; Castellanos, Isabel C; Alvarado, Magda E; Hernández, Paula C; Gallego, Amanda; Wasserman, Moisés.
  • Melo, Sandra P; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Gómez, Vanessa; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Castellanos, Isabel C; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Alvarado, Magda E; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Hernández, Paula C; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Gallego, Amanda; Universidad Nacional de Colombia. Facultad de Ciencias. Departamento de Química. Laboratorio de Investigaciones Básicas en Bioquímica. Bogotá. CO
  • Wasserman, Moisés; Instituto Nacional de Salud. Laboratorio de Bioquímica. Bogotá. CO
Mem. Inst. Oswaldo Cruz ; 103(4): 347-350, June 2008. graf, tab
Article in English | LILACS | ID: lil-486873
ABSTRACT
The reproductive mechanism of Giardia intestinalis, considered one of the earliest divergent eukaryotes, has not been fully defined yet. Some evidence supports the hypothesis that Giardia is an exclusively asexual organism with a clonal population structure. However, the high genetic variability, the variation in ploidy during its life cycle, the low heterozygosity and the existence of genes involved in the meiotic-like recombination pathway in the parasite's genome cast doubt on exclusively asexual nature of Giardia. In this work, semiquantitative RT-PCR analysis was used to assess the transcription pattern of three meiosis-like-specific genes involved in homologues recombination dmc1, hop1 and spo11. The mRNAs were amplified during the parasite's differentiation processes, encystation and excystation, and expression was found at each stage of its life cycle. A semiquantitative assessment also suggests that expression of some of the genes is regulated during encystation process.
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Full text: Available Index: LILACS (Americas) Main subject: Giardia lamblia / Genes, Protozoan / Meiosis Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2008 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Instituto Nacional de Salud/CO / Universidad Nacional de Colombia/CO

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Full text: Available Index: LILACS (Americas) Main subject: Giardia lamblia / Genes, Protozoan / Meiosis Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2008 Type: Article / Project document Affiliation country: Colombia Institution/Affiliation country: Instituto Nacional de Salud/CO / Universidad Nacional de Colombia/CO