Maximum inhibitory dilution of mouthwashes containing chlorhexidine and polyhexamethylene biguanide against salivary staphylococcus aureus
J. appl. oral sci
; 16(5): 336-339, Sept.-Oct. 2008. graf, tab
Article
in En
| LILACS
| ID: lil-495137
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
The aim of the present study was to determine the in vitro maximum inhibitory dilution (MID) of two chlorhexidinebased oral mouthwashes (CHX) Noplak®, Periogard®, and one polyhexamethylene biguanide-based mouthwash (PHMB) Sanifill Premium® against 28 field Staphylococcus aureus strains using the agar dilution method. MATERIALS ANDMETHODS:
For each product, decimal dilutions ranging from 1/10 to 1/655,360 were prepared in distilled water and added to Mueller Hinton Agar culture medium. After homogenization, the culture medium was poured onto Petri dishes. Strains were inoculated using a Steers multipoint inoculator and dishes were incubated at 37ºC for 24hours. For reading, MID was considered as the maximum dilution of the mouthwash still capable of inhibiting microbial growth.RESULTS:
Sanifill Premium® inhibited the growth of all strains at 1/40 dilution and of 1 strain at 1/80 dilution. Noplak® inhibited the growth of 23 strains at 1/640 dilution and of all 28 strains at 1/320 dilution. Periogard® showed inhibited growth of 7 strains at 1/640 dilution and of all 28 strains at 1/320 dilution. Data were submitted to Kruskal-Wallis statistical test, showing significant differences between the mouthwashes evaluated (p<0.05). No significant difference was found between Noplak® and Periogard® (p>0.05). Sanifill Premium® was the least effective (p<0.05).CONCLUSION:
It was concluded that CHX-based mouthwashes present better antimicrobial activity against S. Aureus than the PHMB-based mouthwash.Key words
Full text:
1
Index:
LILACS
Main subject:
Staphylococcus aureus
/
Biguanides
/
Chlorhexidine
/
Anti-Infective Agents, Local
/
Mouthwashes
Language:
En
Journal:
J. appl. oral sci
Journal subject:
ODONTOLOGIA
Year:
2008
Type:
Article