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Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3
Nascimento, Rodrigo Pires do; Alves Junior, Nelson; Coelho, Rosalie Reed Rodrigues.
  • Nascimento, Rodrigo Pires do; Universidade Federal do Recôncavo da Bahia. Cruz das Almas. BR
  • Alves Junior, Nelson; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
  • Coelho, Rosalie Reed Rodrigues; Universidade Federal do Rio de Janeiro. Rio de Janeiro. BR
Braz. j. microbiol ; 42(4): 1384-1389, Oct.-Dec. 2011. ilus
Article in English | LILACS | ID: lil-614599
ABSTRACT
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
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Full text: Available Index: LILACS (Americas) Main subject: Peptide Hydrolases / Saccharomyces cerevisiae / Streptomyces / Actinobacteria / Fermentation Type of study: Evaluation studies Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Recôncavo da Bahia/BR / Universidade Federal do Rio de Janeiro/BR

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Full text: Available Index: LILACS (Americas) Main subject: Peptide Hydrolases / Saccharomyces cerevisiae / Streptomyces / Actinobacteria / Fermentation Type of study: Evaluation studies Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Recôncavo da Bahia/BR / Universidade Federal do Rio de Janeiro/BR