High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Biocell
;
37(1): 1-9, Apr. 2013. ilus, graf
Article
in English
| LILACS
| ID: lil-694715
ABSTRACT
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
Full text:
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Index:
LILACS (Americas)
Main subject:
Oxidants
/
Apoptosis
/
Myoblasts, Skeletal
/
Hydrogen Peroxide
/
Mitochondria
Limits:
Animals
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2013
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional del Sur/AR
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