CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Braz. j. med. biol. res
;
47(4): 273-278, 8/4/2014. graf
Article
in English
| LILACS
| ID: lil-705769
ABSTRACT
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi targeting of CIAPIN1 is a potential approach to reverse MDR of breast cancer. In addition, CIAPIN1 may participate in MDR of breast cancer by regulating P-glycoprotein and P53 expression.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Breast Neoplasms
/
Doxorubicin
/
Drug Resistance, Neoplasm
/
Gene Silencing
/
Intracellular Signaling Peptides and Proteins
/
Antibiotics, Antineoplastic
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2014
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Harbin Medical University/CN
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