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Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans
Cheng, Jianhui; Wang, Hu; Xu, Xin; Wang, Liangyan; Tian, Bing; Hua, Yuejin.
  • Cheng, Jianhui; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
  • Wang, Hu; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
  • Xu, Xin; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
  • Wang, Liangyan; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
  • Tian, Bing; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
  • Hua, Yuejin; Zhejiang Academy of Agricultural Sciences. Institute of Horticulture. Hangzhou. CN
Braz. j. microbiol ; 46(2): 601-611, Apr-Jun/2015. tab, graf
Article in English | LILACS | ID: lil-749726
ABSTRACT
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.
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Full text: Available Index: LILACS (Americas) Main subject: Mutagenesis, Insertional / Deinococcus / Genetic Pleiotropy / Genes, Bacterial Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Zhejiang Academy of Agricultural Sciences/CN

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Full text: Available Index: LILACS (Americas) Main subject: Mutagenesis, Insertional / Deinococcus / Genetic Pleiotropy / Genes, Bacterial Type of study: Prognostic study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2015 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Zhejiang Academy of Agricultural Sciences/CN